Rapamycin causes growth arrest and inhibition of invasion in human chondrosarcoma cells
Rapamycin causes growth arrest and inhibition of invasion in human chondrosarcoma cells
Jian Song1, Xiaobo Wang2, Jiaxue Zhu3, Jun Liu4 1Department of Orthopaedics, Shandong Jining No.1 People’s Hospital, Jining, Shandong, 272000, China; 2Department of Orthopaedics, Wendeng Orthopedic and Traumatic Hospital, Weihai, Shan-dong, 264400, China; 3Department of Orthopaedics, No. 3 People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 201999, China; 4Department of Orthopaedics, Second Affiliated Hospital of Jilin University, Jilin Province, 130000, China
Purpose: Chondrosarcoma is a highly malignant tumor that is characterized by a potent capacity to invade locally and cause distant metastasis and notable for its lack of response to conventional chemotherapy or radiotherapy. Rapamycin, the inhibitor of mammalian target of rapamycin (mTOR), is a valuable drug with diverse clinical applications and regulates many cellular processes. However, the effects of rapamycin on cell growth and invasion of human chondrosarcoma cells are not well known.
Methods: We determined the effect of rapamycin on cell proliferation, cell cycle arrest and invasion by using MTS, flow cytometry and invasion assays in two human chondrosarcoma cell lines, SW1353 and JJ012. Cell cycle regulatory and invasion-related genes’ expression analysis was performed by quantitative RT-PCR (qRT-PCR). We also evaluated the effect of rapamycin on tumor growth by using mice xenograph models.
Results: Rapamycin significantly inhibited the cell proliferation, induced cell cycle arrest and decreased the invasion ability of human chondrosarcoma cells. Meanwhile, rapamycin modulated the cell cycle regulatory and invasion-related genes’ expression. Furthermore, the tumor growth of mice xenograph models with human chondrosarcoma cells was significantly inhibited by rapamycin.
Conclusions: These results provided further insight into the role of rapamycin in chondrosarcoma. Therefore, rapamycin targeted therapy may be a potential treatment strategy for chondrosarcoma.
Key words: cell cycle, cell growth, chondrosarcoma, invasion, rapamycin
Rapamycin causes growth arrest and inhibition of invasion in human chondrosarcoma cells.pdf